isev2
SCIJR7
Erythrocyte haemoglobin-filled ectosomes. Scale bars: 1 μm - grey, 200 nm - white. With permission of co-authors: Božič D, Hočevar M, Kisovec M, Jeran M, Pajnič M, Pađen L, Bedina Zavec A, Podobnik M, Kogej K, Iglič A, Kralj-Iglič V, Stability of haemoglobin-filled erythrocyte vesicles, in preparation. We acknowledge support of Ves4US and ARRS P3-0388, P2-0232, P1-0391 and P2-0132.
We submit an example of transparent image identification of extracellular vesicles. It comprises genesis and the 3D morphology of vesicles. The scanning electron micrographs of a budding erythrocyte (lower right of the upper left panel, please note the narrowing in the distal region of protrusions allowing fission of the budds from the very tips) and an isolate (lower panels) obtained by differential centrifugation from a sample of washed and in-vitro-aged human erythrocytes (stored in phosphate-buffer-saline-citrate at 4°C for one week, vesicle pelleting and washing step performed at 50 000 g, 70 min, 4 °C). Cryo-electron micrograph (upper right panel) reveals the bilayer membrane with electron-dense cargo. Isolate suspended in isotonic (300 mOsm, middle right panel) and hypotonic (50 mOsm, lower two panels) medium was immobilized on a mixed-cellulose-esters filter membrane; different morphology of vesicles can be appreciated.
SCIJR7
Erythrocyte haemoglobin-filled ectosomes. Scale bars: 1 μm - grey, 200 nm - white. With permission of co-authors: Božič D, Hočevar M, Kisovec M, Jeran M, Pajnič M, Pađen L, Bedina Zavec A, Podobnik M, Kogej K, Iglič A, Kralj-Iglič V, Stability of haemoglobin-filled erythrocyte vesicles, in preparation. We acknowledge support of Ves4US and ARRS P3-0388, P2-0232, P1-0391 and P2-0132.
We submit an example of transparent image identification of extracellular vesicles. It comprises genesis and the 3D morphology of vesicles. The scanning electron micrographs of a budding erythrocyte (lower right of the upper left panel, please note the narrowing in the distal region of protrusions allowing fission of the budds from the very tips) and an isolate (lower panels) obtained by differential centrifugation from a sample of washed and in-vitro-aged human erythrocytes (stored in phosphate-buffer-saline-citrate at 4°C for one week, vesicle pelleting and washing step performed at 50 000 g, 70 min, 4 °C). Cryo-electron micrograph (upper right panel) reveals the bilayer membrane with electron-dense cargo. Isolate suspended in isotonic (300 mOsm, middle right panel) and hypotonic (50 mOsm, lower two panels) medium was immobilized on a mixed-cellulose-esters filter membrane; different morphology of vesicles can be appreciated.