Exocytosis of giant vesicles

by Tom Biton

 

Exocytosis is essential in every cell. In exocrine-like tissues, giant

vesicles are “cell-sized” reaching up to 10µm in diameter. In these

systems, an actomyosin coat forcefully squeezes the vesicle’s

content out. The larval salivary gland of Drosophila models this type

of secretion. The video represents a single secretion event where the

cargo of the vesicles, the “glue” proteins are tagged with GFP and

Actin is marked by an expressed actin probe tagged with a Ruby

fluorophore that enables following the formation of the coat. This

super-resolution time-lapse was imaged on a spinning disk confocal

microscope by adapting the SRRF algorithm to super-resolution, live

3D imaging.

Done