2007. Scarified whitebark pine seeds in germination boxes. Dorena Genetic Resource Center. Cottage Grove, Oregon.
Scarified whitebark pine seeds in germination boxes. Dorena Genetic Resource Center. Cottage Grove, Oregon.
Photo by: Richard Sniezko
Date: March 19, 2007
Credit: USDA Forest Service, Region 6, Umpqua National Forest, Dorena Genetic Resource Center.
Source: DRGC digital photo collection; courtesy Richard Sniezko, Cottage Grove, Oregon.
Note:
Scarification and Germination
When stratification is complete, the seedcoat of each seed is abraded, using 100-grit sandpaper, at the radicle end (approximately 1 mm back from the tip) and along the main line dividing the 2 halves of the seedcoat (Figure 5A). Care must be taken to avoid sanding too heavily, as damage to the megagametophyte tissue could result. This process is done to help maximize germination percentage as well as uniformity of germination. Seedlots which are not sanded or nicked show lower germination percentage and germinate at a slower rate (Burr and others 2001). A few seedlots have a percentage of seeds that begin to crack during stratification. These seeds are not sanded.
During the 2007 sowing season, a newly designed sanding machine will be used for seedcoat abrasion (Figure 5B) (Spence 2006). Sanded seeds are placed on moistened blotter paper in 10 x 10 cm clear plastic boxes (Figure 6A), which are then placed in a germinator maintained at 16 °C night/18 °C day with a 12-hour photoperiod (Figure 6B).
Seedlots are monitored for germination twice per week for 5 weeks, and are considered germinated when the radicle protrudes from the seedcoat to a length of 2 mm and is curved (Figure 7).
Excerpted from: Seedling Nursery Culture of Whitebark Pine at Dorena Genetic Resource Center: Headaches, Successes, and Growing Pains. 2007. Riley, Lee E.; Coumas, Carmen M.; Danielson, Judith F.; and Berdeen, John C. Available here: citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.180.659...
Image provided by USDA Forest Service, Region 6, State and Private Forestry, Forest Health Protection: www.fs.usda.gov/main/r6/forest-grasslandhealth
2007. Scarified whitebark pine seeds in germination boxes. Dorena Genetic Resource Center. Cottage Grove, Oregon.
Scarified whitebark pine seeds in germination boxes. Dorena Genetic Resource Center. Cottage Grove, Oregon.
Photo by: Richard Sniezko
Date: March 19, 2007
Credit: USDA Forest Service, Region 6, Umpqua National Forest, Dorena Genetic Resource Center.
Source: DRGC digital photo collection; courtesy Richard Sniezko, Cottage Grove, Oregon.
Note:
Scarification and Germination
When stratification is complete, the seedcoat of each seed is abraded, using 100-grit sandpaper, at the radicle end (approximately 1 mm back from the tip) and along the main line dividing the 2 halves of the seedcoat (Figure 5A). Care must be taken to avoid sanding too heavily, as damage to the megagametophyte tissue could result. This process is done to help maximize germination percentage as well as uniformity of germination. Seedlots which are not sanded or nicked show lower germination percentage and germinate at a slower rate (Burr and others 2001). A few seedlots have a percentage of seeds that begin to crack during stratification. These seeds are not sanded.
During the 2007 sowing season, a newly designed sanding machine will be used for seedcoat abrasion (Figure 5B) (Spence 2006). Sanded seeds are placed on moistened blotter paper in 10 x 10 cm clear plastic boxes (Figure 6A), which are then placed in a germinator maintained at 16 °C night/18 °C day with a 12-hour photoperiod (Figure 6B).
Seedlots are monitored for germination twice per week for 5 weeks, and are considered germinated when the radicle protrudes from the seedcoat to a length of 2 mm and is curved (Figure 7).
Excerpted from: Seedling Nursery Culture of Whitebark Pine at Dorena Genetic Resource Center: Headaches, Successes, and Growing Pains. 2007. Riley, Lee E.; Coumas, Carmen M.; Danielson, Judith F.; and Berdeen, John C. Available here: citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.180.659...
Image provided by USDA Forest Service, Region 6, State and Private Forestry, Forest Health Protection: www.fs.usda.gov/main/r6/forest-grasslandhealth