Ciliate external structure
This is the external structure of a cilliate, likey a paramecium. It was negative stained using Nigrosin to reveal the pelical external structure. The peristome is at the upper right.
A culture rich in ciliates was applied to a microscope slide and 1 drop of 2 % Nigrosin in water was added. The drop was smeared across 2/3 of the slide and let to dry. As the water evaporates, the stain concentrates and most of the ciliates are found in the more concentrated end of the smear.
Imaged using an Olympus BHS with a NEO SPlan 50 NIC objective and using a Sony A7S. This is a metallurgical objective and thus well suited for use without a coverglass.
Ciliate external structure
This is the external structure of a cilliate, likey a paramecium. It was negative stained using Nigrosin to reveal the pelical external structure. The peristome is at the upper right.
A culture rich in ciliates was applied to a microscope slide and 1 drop of 2 % Nigrosin in water was added. The drop was smeared across 2/3 of the slide and let to dry. As the water evaporates, the stain concentrates and most of the ciliates are found in the more concentrated end of the smear.
Imaged using an Olympus BHS with a NEO SPlan 50 NIC objective and using a Sony A7S. This is a metallurgical objective and thus well suited for use without a coverglass.