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Rotifer digestive system

This rotifer (Brachionus sp., I think) was vital stained using 20 ppm Neutral Red perfused from the margin of the coverslip into a well slide. Neutral Red stains lysosomes in living organisms, thus staining the stomach and here its contents. The photo on the left shows the intestinal contents before excretion and at the right after excretion. Specmen from a Northern Indiana lake.

 

Bright field image from a Nikon MS inverted microscope using a 20X DLL objective and a Leica MIKAS adapter to a Sony NEX 5N.

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Uploaded on July 13, 2017
Taken on July 12, 2017