Placental wall in a May Apple
This image shows how the cell morphology of the placental wall in this May Apple gynoexium divers from the bulk cells making up the fruit wall. Hematoxylin has stained the nuclei very dark blue. Brightfieqld image of the longitudinal cross section of the gynoecium from a May Apple (Podophyllum sp.) Specimen from the edge of the Dowagiac River (5/15).
The protocol was as follows. Specimens fixed in FAA (formaldehyde, acetic acid, ethanol) 48 hr. Dehydrated in 35, 50, 75, 85, 95, 99 % IPA in water, 6 hours each min. Infiltrated in xylene saturated with Paraplast for 2 days, followed by 2 changes of melted Praplast for 2 hours each. Embedded in Paraplast. Sectioned on a Spencer 820 microtome at 11 micron. Cleared in Xylene 2X, 10 min each. Rehydrated 99 (10 min), 95, 85, 70 % IPA, 2 min. each. Stained Gill's Hematoxylin 10 sec. Washed 3 min running water. Blued 0.05 % lithium carbonate 3 s. Water rinse 1 min. Stained 1 % aq. Erythrosin-B 2 min. Dehydrated 99 % IPA 2 min. Cleared 2X xyene 5 min each. Mounted with DEPEX.
Photographed on a Spencer 42 petrographic polarizing microscope using an original magnification of 430X, using a Sony NEX 5N with a Leica MIKAS 1/3X adapter.
Placental wall in a May Apple
This image shows how the cell morphology of the placental wall in this May Apple gynoexium divers from the bulk cells making up the fruit wall. Hematoxylin has stained the nuclei very dark blue. Brightfieqld image of the longitudinal cross section of the gynoecium from a May Apple (Podophyllum sp.) Specimen from the edge of the Dowagiac River (5/15).
The protocol was as follows. Specimens fixed in FAA (formaldehyde, acetic acid, ethanol) 48 hr. Dehydrated in 35, 50, 75, 85, 95, 99 % IPA in water, 6 hours each min. Infiltrated in xylene saturated with Paraplast for 2 days, followed by 2 changes of melted Praplast for 2 hours each. Embedded in Paraplast. Sectioned on a Spencer 820 microtome at 11 micron. Cleared in Xylene 2X, 10 min each. Rehydrated 99 (10 min), 95, 85, 70 % IPA, 2 min. each. Stained Gill's Hematoxylin 10 sec. Washed 3 min running water. Blued 0.05 % lithium carbonate 3 s. Water rinse 1 min. Stained 1 % aq. Erythrosin-B 2 min. Dehydrated 99 % IPA 2 min. Cleared 2X xyene 5 min each. Mounted with DEPEX.
Photographed on a Spencer 42 petrographic polarizing microscope using an original magnification of 430X, using a Sony NEX 5N with a Leica MIKAS 1/3X adapter.