Conocephalum sp. epidermis
This is a bright field longitudinal section through the Liverwort Conocephalum sp. showing the epidermal tissue at one of the surface fissures. The Safranin-O has stained the chloroplasts in the cells below the epidermis red.
The protocol was as follows. Specimens fixed in FAA (formaldehyde, acetic acid, ethanol) 24 hr. Dehydrated in IPA at 35, 50, 70, 85, 91, 95, 99 %. Infiltrated with paraplast saturated xylene 48 hr followed by 2 Paraplast baths prior to embedding in Paraplast. Sectioned 11 um thick on a Spencer 820 microtome. Cleared in xylene, 5 min, 2X. Rehydrated in 99, 95, 80, 70 %IPA. Stained in Johansen's Safranin-O, Fast Green (24 hours in Safranin-O, 15 sec. in fast green). Cleared 2X xyene 5 min each. Mounted with DEPEX.
Photographed in bright field on a Spencer 42 petrographic microscope at original magnification of 200X using a Sony NEX-5N and a Leica MIKAS 1/3X adapter.
Conocephalum sp. epidermis
This is a bright field longitudinal section through the Liverwort Conocephalum sp. showing the epidermal tissue at one of the surface fissures. The Safranin-O has stained the chloroplasts in the cells below the epidermis red.
The protocol was as follows. Specimens fixed in FAA (formaldehyde, acetic acid, ethanol) 24 hr. Dehydrated in IPA at 35, 50, 70, 85, 91, 95, 99 %. Infiltrated with paraplast saturated xylene 48 hr followed by 2 Paraplast baths prior to embedding in Paraplast. Sectioned 11 um thick on a Spencer 820 microtome. Cleared in xylene, 5 min, 2X. Rehydrated in 99, 95, 80, 70 %IPA. Stained in Johansen's Safranin-O, Fast Green (24 hours in Safranin-O, 15 sec. in fast green). Cleared 2X xyene 5 min each. Mounted with DEPEX.
Photographed in bright field on a Spencer 42 petrographic microscope at original magnification of 200X using a Sony NEX-5N and a Leica MIKAS 1/3X adapter.