Conocephalum sp. Liverwort pore
This is a bright field longitudinal section through the Liverwort Conocephalum sp at a pore. While not the greatest section, of all the serial sections I prepared, it is the only one clearly showing the pore. The Safranin-O has stained chloroplasts in the layers of cells below the epidermal layers red. Note that the chloroplasts are only near the dorsal surface. I am not sure what the orange stained cells represent.
The protocol was as follows. Specimens fixed in FAA (formaldehyde, acetic acid, ethanol) 24 hr. Dehydrated in IPA at 35, 50, 70, 85, 91, 95, 99 %. Infiltrated with paraplast saturated xylene 48 hr followed by 2 Paraplast baths prior to embedding in Paraplast. Sectioned 11 um thick on a Spencer 820 microtome. Cleared in xylene, 5 min, 2X. Rehydrated in 99, 95, 80, 70 %IPA. Stained in Johansen's Safranin-O, Fast Green (24 hours in Safranin-O, 15 sec. in fast green). Cleared 2X xyene 5 min each. Mounted with DEPEX.
Photographed in bright field on a Spencer 42 petrographic microscope at original magnification of 100X using a Sony NEX-5N and a Leica MIKAS 1/3X adapter.
Conocephalum sp. Liverwort pore
This is a bright field longitudinal section through the Liverwort Conocephalum sp at a pore. While not the greatest section, of all the serial sections I prepared, it is the only one clearly showing the pore. The Safranin-O has stained chloroplasts in the layers of cells below the epidermal layers red. Note that the chloroplasts are only near the dorsal surface. I am not sure what the orange stained cells represent.
The protocol was as follows. Specimens fixed in FAA (formaldehyde, acetic acid, ethanol) 24 hr. Dehydrated in IPA at 35, 50, 70, 85, 91, 95, 99 %. Infiltrated with paraplast saturated xylene 48 hr followed by 2 Paraplast baths prior to embedding in Paraplast. Sectioned 11 um thick on a Spencer 820 microtome. Cleared in xylene, 5 min, 2X. Rehydrated in 99, 95, 80, 70 %IPA. Stained in Johansen's Safranin-O, Fast Green (24 hours in Safranin-O, 15 sec. in fast green). Cleared 2X xyene 5 min each. Mounted with DEPEX.
Photographed in bright field on a Spencer 42 petrographic microscope at original magnification of 100X using a Sony NEX-5N and a Leica MIKAS 1/3X adapter.