Vascular structure near a leaf gall
This is a photomicrograph of a transverse histological cross section of a leaf gall. This is from very near where the gall joins the leaf. A bright field transverse image may be found at: www.flickr.com/photos/14643312@N02/14406464771/in/set-721... . The preparation protocol is given at the bottom. I was not really after this structure when I prepared the slides from serial transverse sections, thus, being near the edge of the actual gall, it did not fair so well during sectioning however it was interesting under polarized light. The cross section of the vascular structure, which the gall shares with the leaf shows some optical activity under polarized light. This is commonly the result of ordered cellulose fiber bundles. These vessels have walls formed in a helical structure with very thin sections between, this explains the banding.
Photographed using a AO Spencer 42 polarizing microscope (petrographic) with crossed polarizers and a lambda wave plate. The Sony NEX 5n was connected to the microscope using a Leica MIKAS microscope adapter with a 2/3X reducer and a 10X eyepiece. The objective was an AO Spencer
Leaf gall 43X.
I prepared this specimen last summer as follows. Fixed in FAA with vacuum-pressure infiltration. Dehydrated 70 % ETOH 95% ETOH 2X, 99% IPA 3X. Cleared in xylene 2X, Infiltrated with Paraplast 2X, mounted in Paraplast. Sectioned with a Spencer 820 microtome 7 micron. Floated on a water drop on a Meyer's Albumin coated slide and dried. Cleared in Xylene 2X. Rehydrated 99% IPA 2X, 90% IPA, 70 % IPA, water. Stained Gill's Hematoxylin III. Running water wash 5 min. Destain 70 % IPA. Stained in Eosin Y, destain 95 % IPA. Dehydrated 99 % IPA 2X. Cleared in Xylene 2X. Mounted with Fisher Permamount.
Vascular structure near a leaf gall
This is a photomicrograph of a transverse histological cross section of a leaf gall. This is from very near where the gall joins the leaf. A bright field transverse image may be found at: www.flickr.com/photos/14643312@N02/14406464771/in/set-721... . The preparation protocol is given at the bottom. I was not really after this structure when I prepared the slides from serial transverse sections, thus, being near the edge of the actual gall, it did not fair so well during sectioning however it was interesting under polarized light. The cross section of the vascular structure, which the gall shares with the leaf shows some optical activity under polarized light. This is commonly the result of ordered cellulose fiber bundles. These vessels have walls formed in a helical structure with very thin sections between, this explains the banding.
Photographed using a AO Spencer 42 polarizing microscope (petrographic) with crossed polarizers and a lambda wave plate. The Sony NEX 5n was connected to the microscope using a Leica MIKAS microscope adapter with a 2/3X reducer and a 10X eyepiece. The objective was an AO Spencer
Leaf gall 43X.
I prepared this specimen last summer as follows. Fixed in FAA with vacuum-pressure infiltration. Dehydrated 70 % ETOH 95% ETOH 2X, 99% IPA 3X. Cleared in xylene 2X, Infiltrated with Paraplast 2X, mounted in Paraplast. Sectioned with a Spencer 820 microtome 7 micron. Floated on a water drop on a Meyer's Albumin coated slide and dried. Cleared in Xylene 2X. Rehydrated 99% IPA 2X, 90% IPA, 70 % IPA, water. Stained Gill's Hematoxylin III. Running water wash 5 min. Destain 70 % IPA. Stained in Eosin Y, destain 95 % IPA. Dehydrated 99 % IPA 2X. Cleared in Xylene 2X. Mounted with Fisher Permamount.