Spindle Gall transverse section
This is a transverse section of a spindle gall found on a leaf of an unidentified shrub found along the Dowagiac River. A photo of the same shrub from last year is at: www.flickr.com/photos/14643312@N02/6965590544. Although not shown in this section the empty sections in the center are where the larval stage of a mite developes. The gall was red before processing. The gall is a growth of the leaf in response to the damage and presence of the egg laying mite. Thus, the structures in the gall are those of the leaf, with the vascular bundles connected to those of the leaf. This is the second histological specimen I have prepared and the first stained using a Hematoxylin-Eosin protocol.
Fixed in FAA with vacuum-pressure infiltration. Dehydrated 70 % ETOH 95% ETOH 2X, 99% IPA 3X. Cleared in xylene 2X, Infiltrated with Paraplast 2X, mounted in Paraplast. Sectioned with a Spencer 820 microtome 7 micron. Floated on a water drop on a Mayer's Albumin coated slide and dried. Cleared in Xylene 2X. Rehydrated 99% IPA 2X, 90% IPA, 70 % IPA, water. Stained Gill's Hematoxylin III. Running water wash 5 min. Destain 70 % IPA. Stained in Eosin Y, destain 95 % IPA. Dehydrated 99 % IPA 2X. Cleared in Xylene 2X. Mounted with Fisher Permount.
Composite image created from 2 bright field micrographs. Photographed using a Sony NEX 5N on a Nikon SM inverted microscope with a Nikon 10X objective, 10X eyepiece and a Leica MICAS microscope adapter with a 2/3X reducer.
Spindle Gall transverse section
This is a transverse section of a spindle gall found on a leaf of an unidentified shrub found along the Dowagiac River. A photo of the same shrub from last year is at: www.flickr.com/photos/14643312@N02/6965590544. Although not shown in this section the empty sections in the center are where the larval stage of a mite developes. The gall was red before processing. The gall is a growth of the leaf in response to the damage and presence of the egg laying mite. Thus, the structures in the gall are those of the leaf, with the vascular bundles connected to those of the leaf. This is the second histological specimen I have prepared and the first stained using a Hematoxylin-Eosin protocol.
Fixed in FAA with vacuum-pressure infiltration. Dehydrated 70 % ETOH 95% ETOH 2X, 99% IPA 3X. Cleared in xylene 2X, Infiltrated with Paraplast 2X, mounted in Paraplast. Sectioned with a Spencer 820 microtome 7 micron. Floated on a water drop on a Mayer's Albumin coated slide and dried. Cleared in Xylene 2X. Rehydrated 99% IPA 2X, 90% IPA, 70 % IPA, water. Stained Gill's Hematoxylin III. Running water wash 5 min. Destain 70 % IPA. Stained in Eosin Y, destain 95 % IPA. Dehydrated 99 % IPA 2X. Cleared in Xylene 2X. Mounted with Fisher Permount.
Composite image created from 2 bright field micrographs. Photographed using a Sony NEX 5N on a Nikon SM inverted microscope with a Nikon 10X objective, 10X eyepiece and a Leica MICAS microscope adapter with a 2/3X reducer.