Dandelion stem
Photomicrograph of a Dandelion stem cross section. This is my first attempt at histological specimen preparation. Next time I need to use a thinner layer of Meyer's Albumin as it took up some of the stain.
Bright field micrograph created using a Sony NEX 5N on a Nikon SM inverted microscope with a Zeiss 2.5X objective, 4X eyepiece and a Leica MICAS microscope adapter with a 2/3X reducer.
Fixed in FAA 48 hr. Dehydrated 70 % ETOH 95% ETOH 2X, 99% IPA 3X. Cleared in xylene 2X, Infiltrated with Paraplast 2X, mounted in Paraplast. Sectioned with a Spencer 820 microtome at 7 microns. Floated on a water drop on a Mayer's Albumin coated slide and dried. Cleared in Xylene 2X. Rehydrated 99% IPA, 95% ETOH. Stained with Aniline Blue IPA, fixed acid alcohol, stained Orange G. Dehydrated 95% ETOH, 99 % IPA 2X. Cleared in Xylene 2X. Mounted with Fisher Permount.
Dandelion stem
Photomicrograph of a Dandelion stem cross section. This is my first attempt at histological specimen preparation. Next time I need to use a thinner layer of Meyer's Albumin as it took up some of the stain.
Bright field micrograph created using a Sony NEX 5N on a Nikon SM inverted microscope with a Zeiss 2.5X objective, 4X eyepiece and a Leica MICAS microscope adapter with a 2/3X reducer.
Fixed in FAA 48 hr. Dehydrated 70 % ETOH 95% ETOH 2X, 99% IPA 3X. Cleared in xylene 2X, Infiltrated with Paraplast 2X, mounted in Paraplast. Sectioned with a Spencer 820 microtome at 7 microns. Floated on a water drop on a Mayer's Albumin coated slide and dried. Cleared in Xylene 2X. Rehydrated 99% IPA, 95% ETOH. Stained with Aniline Blue IPA, fixed acid alcohol, stained Orange G. Dehydrated 95% ETOH, 99 % IPA 2X. Cleared in Xylene 2X. Mounted with Fisher Permount.