Replication Forks
We're here is studying and exploring reflectionism and related
palindromic phenomena.
My idea was to show palindromes in genetics. Unfortunately my electron microscope is in the repair shop, so I had to use some ordinary kitchen forks to illustrate the concept.
Learn more about palindromes as substrates for multiple pathways of recombination in Escherichia coli.
Long DNA palindromes are sites of genome instability (deletions, amplification, and translocations) in both prokaryotic and eukaryotic cells. In Escherichia coli, genetic evidence has suggested that they are sites of DNA cleavage by the SbcCD complex that can be repaired by homologous recombination. Here we obtain in vivo physical evidence of an SbcCD-induced DNA double-strand break (DSB) at a palindromic sequence in the E. coli chromosome and show that both ends of the break stimulate recombination. Cleavage is dependent on DNA replication, but the observation of two ends at the break argues that cleavage does not occur at the replication fork. Genetic analysis shows repair of the break requires the RecBCD recombination pathway and PriA, suggesting a mechanism of bacterial DNA DSB repair involving the establishment of replication forks.
(Science Direct; SbcCD Causes a Double-Strand Break at a DNA Palindrome in the Escherichia coli Chromosome; John K.Eykelenboom, John K.Blackwood, EwaOkely, David R.F.Leach)
www.sciencedirect.com/science/article/pii/S1097276508000397
Replication Forks
We're here is studying and exploring reflectionism and related
palindromic phenomena.
My idea was to show palindromes in genetics. Unfortunately my electron microscope is in the repair shop, so I had to use some ordinary kitchen forks to illustrate the concept.
Learn more about palindromes as substrates for multiple pathways of recombination in Escherichia coli.
Long DNA palindromes are sites of genome instability (deletions, amplification, and translocations) in both prokaryotic and eukaryotic cells. In Escherichia coli, genetic evidence has suggested that they are sites of DNA cleavage by the SbcCD complex that can be repaired by homologous recombination. Here we obtain in vivo physical evidence of an SbcCD-induced DNA double-strand break (DSB) at a palindromic sequence in the E. coli chromosome and show that both ends of the break stimulate recombination. Cleavage is dependent on DNA replication, but the observation of two ends at the break argues that cleavage does not occur at the replication fork. Genetic analysis shows repair of the break requires the RecBCD recombination pathway and PriA, suggesting a mechanism of bacterial DNA DSB repair involving the establishment of replication forks.
(Science Direct; SbcCD Causes a Double-Strand Break at a DNA Palindrome in the Escherichia coli Chromosome; John K.Eykelenboom, John K.Blackwood, EwaOkely, David R.F.Leach)
www.sciencedirect.com/science/article/pii/S1097276508000397