plosone-phylo
Figure 1
Alignment of amino acid sequences and phylogenetic analysis of the IbDFR protein with other species.(A) Multiple alignment of deduced amino acid sequences of dihydroflavonol-4-reductase (DFR) from sweet potato, Arabidopsis and Ipomoea purpurea. The black and other shaded boxes show identical and similar amino acids, respectively. The underline indicates the putative NADP binding site. (B) A phylogenetic tree of known plant DFR for anthocyanin biosynthesis. The GenBank accession numbers are: Arabidopsis thaliana (AtDFR, BAD95233), Fragaria ananassa (FaDFR, AAC25960), Malus domestica (MdDFR, AAD26204), Vitis vinifera (VvDFR, XP_002281858), Dianthus caryophyllus (DcDFR, CAA91924), Gerbera hybrid (GhDFR, CAA78930), Gentiana triflora (GtDFR, BAA12736), Ipomoea purpurea (IpDFR, BAA74700), Nicotiana tabacum (NtDFR, ABN80437), Solanum lycopersicum (SlDFR, CAA79154), Vaccinium macrocarpon (VmDFR, AAL89714), Hordeum vulgare (HvDFR, AAB20555), Bromheadia finlaysoniana (BfDFR, AAB62873), Ginkgo biloba (GbDFR, AAU95082), Antirrhinum majus (AmDFR, P14721), Medicago truncatula (MtDFR1, AAR27014), Populus tremuloides (PtDFR, AY147903), Solanum tuberosum (StDFR, AEN83503). The horizontal scale shows the number of differences per 100 residues derived from Clustal W alignment.
Figure 1
Alignment of amino acid sequences and phylogenetic analysis of the IbDFR protein with other species.(A) Multiple alignment of deduced amino acid sequences of dihydroflavonol-4-reductase (DFR) from sweet potato, Arabidopsis and Ipomoea purpurea. The black and other shaded boxes show identical and similar amino acids, respectively. The underline indicates the putative NADP binding site. (B) A phylogenetic tree of known plant DFR for anthocyanin biosynthesis. The GenBank accession numbers are: Arabidopsis thaliana (AtDFR, BAD95233), Fragaria ananassa (FaDFR, AAC25960), Malus domestica (MdDFR, AAD26204), Vitis vinifera (VvDFR, XP_002281858), Dianthus caryophyllus (DcDFR, CAA91924), Gerbera hybrid (GhDFR, CAA78930), Gentiana triflora (GtDFR, BAA12736), Ipomoea purpurea (IpDFR, BAA74700), Nicotiana tabacum (NtDFR, ABN80437), Solanum lycopersicum (SlDFR, CAA79154), Vaccinium macrocarpon (VmDFR, AAL89714), Hordeum vulgare (HvDFR, AAB20555), Bromheadia finlaysoniana (BfDFR, AAB62873), Ginkgo biloba (GbDFR, AAU95082), Antirrhinum majus (AmDFR, P14721), Medicago truncatula (MtDFR1, AAR27014), Populus tremuloides (PtDFR, AY147903), Solanum tuberosum (StDFR, AEN83503). The horizontal scale shows the number of differences per 100 residues derived from Clustal W alignment.