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Evolutionary tree analysis of the thiolase sequences.The phylogenetic tree was constructed using the neighbor-joining method, with 10,000 bootstrap replicates in MEGA5 software. Only the region corresponding to the thiolase domain was used for these calculations. The group identifiers correspond to the nomenclature described in the text. The numbers next to each node indicate bootstrap values as percentages. The following sequences (listed with their NCBI accession codes and, for trypanosomatid sequences, their NCBI and GeneDB accession codes) were used for creating the evolutionary tree. 1, Human: HsT1 (NP_006102.2); 2, Mus musculus: MmT1 (NP_803421.1); 3, Salmo salar: SsT1 (ACI33809.1); 4, Monosiga brevicollis: MbT1 (XP_001748310.1); 5, Bdellovibrio bacteriovorus: BbT1 (NP_967398.1); 6, Zoogloea ramigera: ZrCT (1DM3); 7, HsCT (NP_005882.2); 8, MmCT (NP_033364.2); 9, HsT2 (NP_000010.1); 10, Macaca fascicularis: MfT2 (Q8HXY6.1); 11, Rattus norvegicus: RnT2 (NP_058771.1); 12, MmT2 (NP_659033.1); 13, Saccharomyces cerevisiae: ScAB (1AFW); 14, HsAB (NP_001598.1); 15, Arabidopsis thaliana: AtAB (2WU9); 16, T. cruzi: TcAB (Tc00.1047053511003.60, XP_816035.1); 17, Bodo saltans: BsAB (BSA00133, ACI16032.1); 18, HsTFE (NP_000174.1); 19, TcTFE (Tc00.1047053511389.150, XP_814301.1); 20, L. braziliensis: LbTFE (LbrM.31.1840, XP_001567180.1); 21, L. major: LmTFE (LmjF.31.1640, XP_001685150.1); 22, L. mexicana: LxTFE (LmxM.30.1640.1, CBZ29221.1); 23, L. infantum: LiTFE (LinJ.31.1660, CAM70518.2); 24, HsSCP2 (NP_002970.2); 25, LmSCP2 (LmjF.23.0690, XP_001683404.1); 26, LiSCP2 (LinJ.23.0860, XP_001465761.1); 27, LxSCP2 (LmxM.23.0690.1, CBZ27218.1); 28, LbSCP2 (LbrM.23.0840, XP_001565156.1); 29, TcSCP2 (Tc00.1047053510507.20, XP_807246.1); 30, T. vivax: TvSCP2 (TvY486_0802010); 31, T. congolense: ToSCP2 (congo1030g10.p1k_8); 32, T. brucei: TbSCP2 (Tb927.8.2540, XP_847087.1); 33, T. gambiense: TgSCP2 (Tbg972.8.2020); 34, M. smegmatis: MsSTLP2 (YP_887911.1); 35, M. tuberculosis: MtSTLP (NP_216383.1); 36, R. jostii: RjSTLP (YP_700393.1); 37, C. crescentus: CcSTLP (YP_002517418.1); 38, H. neptunium: HnSTLP (YP_759708.1); 39, MsTLP1 (YP_889758.1); 40, Erythrobacter sp.: EbSTLP (ZP_01041346.1).

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Uploaded on May 6, 2014
Taken on May 6, 2014