plosone-phylo
pone.0006367.g002.png
L-gene phylogeny with novel bat paramyxoviruses.
The phylogenetic tree was generated and annotated using BEAST version 1.4.8, using a codon-based substitution and an expansion growth population model [31]. For better graphic visualization, only posterior probability values below 1.0 are shown. The initial sequence alignment was based on a 496 base pair fragment from PCR screening. Exclusion of primer sites resulted in a 439 bp fragment, and further exclusion of ambiguous sites resulted in a 426 base pairs gap-free alignment corresponding to nucleotides 9993–10430 of Human parainfluenza virus 1 strain Washington/1964, GenBank Accession Number NC_003461. Novel bat paramyxoviruses are shown in boldface type. GenBank Accession numbers of the novel viruses are FJ609191 (BatPV/Eid.hel/GH10/2008), FJ609194 (BatPV/Eid.hel/GH48/2008) and GQ168929 (BatPV/Eid.hel/GH45/2008). Branches leading to the Henipavirus genus are in red color. Established genera in the Paramyxoviridae family are indicated next to taxon names. Taxa are named according to the following pattern: Genus/typical host/virus abbreviation/accession number/isolation year. HenipaV = Henipavirus, MorbilliV = Morbillivirus, RespiroV = Respirovirus, RubulaV = Rubulavirus, AvulaV = Avulavirus, MetapneumoV = Metapneumovirus, PneumoV = Pneumovirus, UPV = unclassified paramyxovirus. NiV = Nipah virus, HeV = Hendra virus, PPRV = Peste-des-petitsruminants virus, CeMV DMV = Cetacean morbillivirus strain dolphin morbillivirus, MeV = Measles virus, RPV = Rinderpest virus, PDV = Phocine distemper virus, CDV = Canine distemper virus, SeV = Sendai virus, bPIV3 = Bovine parainfluenza virus 3, hPIV1 = Human parainfluenza virus 1, hPIV3 = Human parainfluenza virus 3, SV5 = Simian parainfluenza virus 5, SV41 = Simian virus 41, MenPV = Menangle virus, MprPV = Mapuera virus, MuV = Mumps virus, PorPV = Porcine rubulavirus, TioPV = Tioman virus, hPIV2 = Human parainfluenza virus 2, aMPV4 = Avian paramyxovirus type 4, aMPV6 = Avian paramyxovirus type 6, aMPV9 = Avian paramyxovirus type 9, aMPV2 = Avian paramyxovirus type 2, aMPV3 = Avian paramyxovirus type 3, NDV = Newcastle disease virus, hMPV = Human metapneumovirus, aMPV = Avian metapneumovirus, MPV = Murine pneumonia virus, bRSV = Bovine respiratory syncytial virus, hRSV = Human respiratory syncytial virus, NarPV = Nariva virus, ASPV = Atlantic salmon paramyxovirus, TuPV = Tupaia paramyxovirus, MosPV = Mossman virus, BeiPV = Beilong virus, JPV = J virus, FdlPV = Fer-de-lance virus.
pone.0006367.g002.png
L-gene phylogeny with novel bat paramyxoviruses.
The phylogenetic tree was generated and annotated using BEAST version 1.4.8, using a codon-based substitution and an expansion growth population model [31]. For better graphic visualization, only posterior probability values below 1.0 are shown. The initial sequence alignment was based on a 496 base pair fragment from PCR screening. Exclusion of primer sites resulted in a 439 bp fragment, and further exclusion of ambiguous sites resulted in a 426 base pairs gap-free alignment corresponding to nucleotides 9993–10430 of Human parainfluenza virus 1 strain Washington/1964, GenBank Accession Number NC_003461. Novel bat paramyxoviruses are shown in boldface type. GenBank Accession numbers of the novel viruses are FJ609191 (BatPV/Eid.hel/GH10/2008), FJ609194 (BatPV/Eid.hel/GH48/2008) and GQ168929 (BatPV/Eid.hel/GH45/2008). Branches leading to the Henipavirus genus are in red color. Established genera in the Paramyxoviridae family are indicated next to taxon names. Taxa are named according to the following pattern: Genus/typical host/virus abbreviation/accession number/isolation year. HenipaV = Henipavirus, MorbilliV = Morbillivirus, RespiroV = Respirovirus, RubulaV = Rubulavirus, AvulaV = Avulavirus, MetapneumoV = Metapneumovirus, PneumoV = Pneumovirus, UPV = unclassified paramyxovirus. NiV = Nipah virus, HeV = Hendra virus, PPRV = Peste-des-petitsruminants virus, CeMV DMV = Cetacean morbillivirus strain dolphin morbillivirus, MeV = Measles virus, RPV = Rinderpest virus, PDV = Phocine distemper virus, CDV = Canine distemper virus, SeV = Sendai virus, bPIV3 = Bovine parainfluenza virus 3, hPIV1 = Human parainfluenza virus 1, hPIV3 = Human parainfluenza virus 3, SV5 = Simian parainfluenza virus 5, SV41 = Simian virus 41, MenPV = Menangle virus, MprPV = Mapuera virus, MuV = Mumps virus, PorPV = Porcine rubulavirus, TioPV = Tioman virus, hPIV2 = Human parainfluenza virus 2, aMPV4 = Avian paramyxovirus type 4, aMPV6 = Avian paramyxovirus type 6, aMPV9 = Avian paramyxovirus type 9, aMPV2 = Avian paramyxovirus type 2, aMPV3 = Avian paramyxovirus type 3, NDV = Newcastle disease virus, hMPV = Human metapneumovirus, aMPV = Avian metapneumovirus, MPV = Murine pneumonia virus, bRSV = Bovine respiratory syncytial virus, hRSV = Human respiratory syncytial virus, NarPV = Nariva virus, ASPV = Atlantic salmon paramyxovirus, TuPV = Tupaia paramyxovirus, MosPV = Mossman virus, BeiPV = Beilong virus, JPV = J virus, FdlPV = Fer-de-lance virus.