Figure 1

Phylogenetic tree construction and multiple sequence alignment of Cactus proteins from various species.(A) Neighbor-joining phylogenetic tree analysis of the full-length amino acid sequences of Cactus proteins (LvCactus was marked with solid triangle) using MEGA 5.0 software; (B) Schematic representation and (C) Multiple sequence alignment (using clustal X v2.0 method) of the ankyrin repeat domains of Cactus proteins with the identical amino acid residues shaded in black and the similar residues in gray. Proteins analyzed list below: LvCactus, Litopenaeus vannamei Cactus (Accession No. JX014314); AmCactus, Apis mellifera Cactus 1 (Accession No. NP_001157184.1); AeCactus, Acromyrmex echinatior Cactus (Accession No. EGI65248.1); BmCactus, Bombyx mori Cactus (Accession No. NP_001166191.1); CfCactus, Camponotus floridanus Cactus (Accession No. EFN66754.1); CeIκB-1, Caenorhabditis elegans IκB-1 (Accession No. NP_492575.1); DmCactus1, Drosophila melanogaster Cactus isoform A (Accession No. AAN10936.1); DmCactus2, Drosophila melanogaster Cactus isoform B (Accession No. NP_476942.1); DpCactus, Daphnia pulex Cactus (Accession No. EFX89207.1); HsIκB Alpha, Homo sapiens IκB Alpha (Accession No. NP_065390.1); HsIκB beta1, Homo sapiens IκB beta isoform 1 (Accession No. NP_002494.2); HsIκB beta2, Homo sapiens IκB beta isoform 2 (Accession No. NP_001230045.1); HsIκB Epsilon, Homo sapiens IκB Epsilon (Accession No. NP_004547.2); HsIκB Zeta a, Homo sapiens IκB Zeta isoform a (Accession No. NP_113607.1); HsIκB Zeta b, Homo sapiens IκB Zeta isoform b (Accession No. NP_001005474.1); HsIκB Delta, Homo sapiens IκB Delta (Accession No. NP_640332.1); TcCactus1, Tribolium castaneum Cactus isoform 1 (Accession No. NP_001157183.1) and TcCactus2, Tribolium castaneum Cactus isoform 2 (Accession No. NP_001157182.1).

Done