plosone-phylo
pone.0035329.g002
Comparative analysis of volumetric asymmetry in the habenulae of teleosts.(A) Drawings of adult male individuals belonging to different teleost species, placed in the context of a cladogram of the teleost lineage according to Nelson [28]. For each species, the corresponding panels from columns B to F are aligned horizontally. (B) Schematics of habenular cytoarchitecture obtained from the cresyl-violet stained coronal sections of the Hb shown in Figure 1. (C) Volumetric models of the Hb as seen dorsally, with anterior to the top. Volumes of the left and right Hb have been differentially coloured in red and green, respectively. (D) Colour code diagram indicating the presence of statistically significant laterality of habenular volume at the population level ([R-L]≠0). For each box, the presence of left- or right- directional asymmetries, and the corresponding p values, have been coloured according to the colour scale given below. Vertical lines and asterisks placed on the left side of some pairs of boxes indicate sex-specific significant differences in habenular asymmetry (* = p<0.05; ** = p<0.01). (E) Box plots indicating the scores of habenular Laterality Index for each species and sex. Positive and negative values indicate right- and a left- sided laterality of habenular volume, respectively. Values reveal the strength and directionality of habenular asymmetry at the population level. The vertical line with asterisks placed indicates sex-specific significant differences in Laterality Index (** = p<0.01). (F) Box plots indicating the absolute values of Laterality Index (abs-LI) for each species and sex, which reveal the strength of habenular asymmetry at the individual level. For each plot shown in E and F, the box depicts the interquartile range containing 50% of the data around the median (vertical line inside the box), and the whisker depicts maximum and minimum vales. Abbreviations: A (anterior), Asym (asymmetry), Cyto (cytoarchitecture), D (dorsal), F (females), L (left), M (males), P (posterior), R (right), V (ventral). Scale bar: 100 µm for column B, and 200 µm for column C.
pone.0035329.g002
Comparative analysis of volumetric asymmetry in the habenulae of teleosts.(A) Drawings of adult male individuals belonging to different teleost species, placed in the context of a cladogram of the teleost lineage according to Nelson [28]. For each species, the corresponding panels from columns B to F are aligned horizontally. (B) Schematics of habenular cytoarchitecture obtained from the cresyl-violet stained coronal sections of the Hb shown in Figure 1. (C) Volumetric models of the Hb as seen dorsally, with anterior to the top. Volumes of the left and right Hb have been differentially coloured in red and green, respectively. (D) Colour code diagram indicating the presence of statistically significant laterality of habenular volume at the population level ([R-L]≠0). For each box, the presence of left- or right- directional asymmetries, and the corresponding p values, have been coloured according to the colour scale given below. Vertical lines and asterisks placed on the left side of some pairs of boxes indicate sex-specific significant differences in habenular asymmetry (* = p<0.05; ** = p<0.01). (E) Box plots indicating the scores of habenular Laterality Index for each species and sex. Positive and negative values indicate right- and a left- sided laterality of habenular volume, respectively. Values reveal the strength and directionality of habenular asymmetry at the population level. The vertical line with asterisks placed indicates sex-specific significant differences in Laterality Index (** = p<0.01). (F) Box plots indicating the absolute values of Laterality Index (abs-LI) for each species and sex, which reveal the strength of habenular asymmetry at the individual level. For each plot shown in E and F, the box depicts the interquartile range containing 50% of the data around the median (vertical line inside the box), and the whisker depicts maximum and minimum vales. Abbreviations: A (anterior), Asym (asymmetry), Cyto (cytoarchitecture), D (dorsal), F (females), L (left), M (males), P (posterior), R (right), V (ventral). Scale bar: 100 µm for column B, and 200 µm for column C.