Figure 2

Phylogenetic analyses showing the unique status of plathyhelminth ?-integrins.Phylogram of the analysis of the full-length sequences of the S. mansoni ?-integrin receptors Sm?-Int1, Sm?-Int2, Sm?-Int3, and other ?-integrin receptors using CLUSTAL X (www.clustal.org) and TreeViewX. The phylogenetic relationship was deduced using the Bootstrap Neighbour-Joining (N?J) method and the bootstrap values were generated based on 1000 bootstrap trails with a random number generator seed of 100. Sequences were obtained from the National Centre for Biotechnology Information using the WWW Entrez Browser (www.ncbi.nlm.nih.gov), Swiss-Prot (www.uniprot.org), GeneDB (www.genedb.org), and the Schmidtea mediterranea Genome Database (smedgd.neuro.utah.edu/). The corresponding protein numbers are: Sha a1 (?-integrin 1 receptor, S. haematobium; Sha_102401), Sm a1 (?-integrin 1 receptor, S. mansoni; FR749887), Sjp a1 (?-integrin 1 receptor, S. japonicum; Sjp_0037690), Cs a5 (?-integrin 5 receptor, Clonorchis sinesis; GAA56616.1), Em a1 (?-integrin 1 receptor, Echinococcus multilocularis; EmuJ_000215000 ), Sm a4 (?-integrin 4 receptor, S. mansoni; Smp_1735401, Smp_181010), Sha a4 (?-integrin 4 receptor, S. haematobium; Sha_104436, Sha_106831), Sjp a4 (?-integrin 4 receptor, S. japonicum; Sjp_0046780, Sjp_0046790), Cs a4 (?-integrin 4 receptor, Clonorchis sinesis; GAA28731), Em a4 (?-integrin 4 receptor, Echinococcus multilocularis; EmuJ_000573500), Sha a2 (?-integrin 2 receptor, S. haematobium; Sha_106921), Sm a2 (?-integrin 2 receptor, S. mansoni; FR749888), Sjp a2 (?-integrin 2 receptor, S. japonicum; Sjp_0069490), Cs a-ps (?-integrin-ps receptor, Clonorchis sinesis; GAA54095, GAA49531, GAA49530), Em a2 (?-integrin 2 receptor, Echinococcus multilocularis; EmuJ_000192500 ), Smed a3 (?-integrin 3 receptor, Schmidtea mediterranea; lcl|mk4.000046.14.01), Smed a1 (?-integrin 1 receptor, Schmidtea mediterranea; lcl|mk4.001411.00.01), Smed a2 (?-integrin 2 receptor, Schmidtea mediterranea; lcl|mk4.003797. 00.01), Sha a3 (?-integrin 3 receptor, S. haematobium; Sha_102914), Sm a3 (?-integrin 3 receptor, S. mansoni; FR749889, Smp_156610, Smp_156620), Sjp a3 (?-integrin 3 receptor, S. japonicum; Sjp_0063430, Sjp_0063420), Cs a7 (?-integrin 7 receptor, Clonorchis sinesis; GAA52225.1), Em a3 (?-integrin 3 receptor, Echinococcus multilocularis; EmuJ_000782500), Sp aP (?-integrin P receptor, Strongylocentrotus purpuratus, AF177914), Dm aPS2 (?-integrin PS2 receptor, Drosophila melanogaster, Q24247), Mm a2b (?-integrin 2b receptor, Mus musculus; EDL34136.1), Hs a2b (?-integrin 2b receptor, Homo sapiens; EAW51595.1), Xl a2b (?-integrin 2b receptor, Xenopus laevis; NP_001088223.1), Mm a5 (?-integrin 5 receptor, Mus musculus; CAA55638.1), Rn a5 (?-integrin 5 receptor, Rattus norvegicus; NP_001101588.1), Hs a5 (?-integrin 5 receptor, Homo sapiens; NP_002196.2), Xl a5 (?-integrin 5 receptor, Xenopus laevis; NP_001081072.1), Hs aV (?-integrin V receptor, Homo sapiens; P06756), Hs a8 (?-integrin 8 receptor, Homo sapiens; P53708), Ce a-pat2 (?-integrin pat-2, Ceanorhabditis elegans; P34446), Gc a (?-integrin receptor, Geodia cydonium; X97283), Hs a1 (?-integrin 1 receptor, Homo sapiens; P56199), Hs a2 (?-integrin 2 receptor, Homo sapiens; P17301), Hs a10 (?-integrin 10 receptor, Homo sapiens; O75578), Hs a11 (?-integrin 11 receptor, Homo sapiens; Q9UKX5), Hs aD (?-integrin D receptor, Homo sapiens; Q13349), Hs aX (?-integrin X receptor, Homo sapiens; P20702), Hs aM (?-integrin M receptor, Homo sapiens; P11215), Hs aL (?-integrin L receptor, Homo sapiens; P20701), Hs aE (?-integrin E receptor, Homo sapiens; P38579), Hs a4 (?-integrin 4 receptor, Homo sapiens; P13612), Hs a9 (?-integrin 9 receptor, Homo sapiens; Q13797), Mm a7 (?-integrin 7 receptor, Mus musculus; AAA16600.1), Rn a7 (?-integrin 7 receptor, Rattus norvegicus; NP_110469.1), Hs a7 (?-integrin 7 receptor, Homo sapiens; EAW96822.1), Hs a6 (?-integrin 6 receptor, Homo sapiens; P23229), Hs a3 (?-integrin 3 receptor, Homo sapiens; P26006), Dm aPSI (?-integrin PSI receptor, Drosophila melanogaster, Q24247), and Ce a-ina1 (?-integrin ina1, Ceanorhabditis elegans; Q03600).

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