Figure 1

Characterization and phylogenetic analysis of HoblOBP3 and HoblOBP4.(A-B) The ORF of nucleotide sequence and deduced amino acid sequence of the OBP3 (A) and OBP4 (B) from H. oblita. The six conserved cysteines are indicated in rings with red color. The predicted signal peptide is underlined. The asterisk with red color marks the translation-termination codon. (C) Alignment of some OBPs amino acid sequence from Coleoptera insects. (D) Alignment of amino acid sequence between HoblOBP3 and HoblOBP4. (E) Phylogenetic tree of OBPs amino acid sequences in Coleoptera, including HoblOBPs. The corresponding OBPs in alignment and phylogenetic tree are listed as follow. RpalOBP2 (AAD31875, Rhynchophorus palmarum), DponOBP (AFI45058, Dendroctonus ponderosae), TcasOBP09 (EFA10713, Tribolium castaneum), TcasOBP23 (EFA10803, Tribolium castaneum), TcasOBP07 (EFA04593, Tribolium castaneum), HparOBP (AEA76516, Holotrichia parallela), HparPBP1 (ADF87391, Holotrichia parallela), HparOBP1 (BAC07272, Holotrichia parallela), HpicOBP1(BAC07270, Heptophylla picea), HpicOBP2 (BAC07271, Heptophylla picea), PjapPBP (AAC63436, Popillia japonica), EoriPBP (BAB70711, Exomala orientalis), AosaPBP (AAC63437, Anomala osakana), PdivOBP1 (BAA88061, Phyllopertha diversa), PdivOBP2(BAA88062, Phyllopertha diversa), AcupPBP1 (BAC06496, Anomala cuprea), ArufPBP (BAF79995, Anomala rufocuprea), ArufPBP2 (BAF91329, Anomala rufocuprea), AschPBP(BAF79599, Anomala schonfeldti), AschPBP2 (BAF79600, Anomala schonfeldti), MaltOBP1 (ABR53888, Monochamus alternatus), HoblOBP1(ACX32050, Holotrichia oblita), HoblOBP2(ACX32049, Holotrichia oblita), HoblOBP3 (ADX96030, Holotrichia oblita), HoblOBP4 (ADX96031, Holotrichia oblita).

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