Segmentation of GFP based calcium sensor fluorescence in the C. elegans spermatheca.

by Jeff Bouffard and Erin J. Cram

 

Still frame from a calcium sensor movie of the Caenorhabditis

elegans spermatheca, with outputs from a fluorescence

segmentation program. Top left: Raw fluorescence from the GFP

based calcium sensor GCaMP, under the control of a spermatheca

specific promoter. Top right: Intensities of initial segmentation

boundaries. Bottom left: Initial segmentation boundaries calculated in

three different directions, color-coded as red, green, and blue.

Compound colors, i.e. yellows, magentas, and whites, display

overlaps that indicate high confidence segmentation boundaries.

Bottom right: Refined segmentation boundaries in white, overlaid on

the GCaMP fluorescence frame false colored with the ImageJ Fire

look up table. Segmentation of calcium sensor fluorescence helps us

refine our measurements to understand how cells coordinate to

generate tissue level calcium signaling and tissue function. Bar =

10μm.

Done