LifeInMegapixels
Double lens reverse microphotography
a trial of using double lens reverse macro technique for microphotography on a cross section of a plant root.
55-250IS@250mm on 500D body with 18-55IS@18mm reverse mounted. This was mounted above an old microscope (with viewing optics removed) to use the mount and light for lighting and micro adjustement of focus. Shake is a major issue and this set up requires alot of light, so even with the microscope illumination, i used both lenses wide open, ISO 6400, Av with -1Ev for 1/320s exposure.
lens set up gives a calculated magnification of 250/18=13.8x. On a canon APS-C 1.6x crop factor this gives a FOV of approximately 1.6mm. After softening and noise, resolution is a little shy of the micrometer range.
Double lens reverse microphotography
a trial of using double lens reverse macro technique for microphotography on a cross section of a plant root.
55-250IS@250mm on 500D body with 18-55IS@18mm reverse mounted. This was mounted above an old microscope (with viewing optics removed) to use the mount and light for lighting and micro adjustement of focus. Shake is a major issue and this set up requires alot of light, so even with the microscope illumination, i used both lenses wide open, ISO 6400, Av with -1Ev for 1/320s exposure.
lens set up gives a calculated magnification of 250/18=13.8x. On a canon APS-C 1.6x crop factor this gives a FOV of approximately 1.6mm. After softening and noise, resolution is a little shy of the micrometer range.