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Bayesian phylogenetic trees derived from 31 global samples of DENV-3 based on the 3′UTR sequences inferred with MrBayes program.

The posterior probabilities expressed in percent are indicated key nodes. The DENV-1 (M87512) and DENV4 (AY618992) strains were used as outgroup. Horizontal branch lengths are drawn to scale. Aligned sequences were analyzed in the MrModeltest 2.3 program to identify the best fit-model of nucleotide substitution for Bayesian phylogenetic reconstruction. The nucleotide substitution model used was under a General Time Reversible model of nucleotide substitution with a proportion of invariable sites (I = 0.6264) (GTR+I), using Akaike's Information Criterion (AIC). Five runs of 4 chains each (one cold and tree heated, temperature = 0.20) were run for 1.5×106 generations, with a burn-in of 6000 generations. GenBank accession numbers: D3BR PV7 03 (FJ481175), In_98901437_DSS_DV_3_98 (AB189126), In_98901403_DSS_DV_3_98 (AB189125), ET_D3_Hu_TL018NIID_2005 (AB214879), ET_D3_Hu_TL029NIID_2005 (AB214880), China_80_2_(AF317645), D3_H_IMTSSA_MART_1999_1243 (AY099337), D3_H_IMTSSA_SRI_2000_1266 (AY099336), BR74886_02 (AY679147), Philip56_H87 (M93130), BR_DEN3_95_04 (EF629366), BR_DEN3_290_02 (EF629369), BR_DEN3_RO1_02 (EF629370), BR_DEN3_RO2_02_(EF629373), D3_SG_SS710_2004 (EU081181), Singapore (AY662691), PtoR_BID_V859_1998 (EU482596), PtoR_BID_V1088_1998 (EU482566), PtoR_BID_V1078_2003 (EU482564), PtoR_BID_V1075_1998 (EU482563), PtoR_BID_V1043_2006 (EU482555), PtoR_BID_V1049_1998 (EU482558), PtoR_BID_V1050_1998 (EU482559), VEN_BID_V904_2001 (EU482612), PhMH_J1_97 (DQ401695), BDH02_1_02 (AY496871), BDH02_3_02 (AY496873), BDH02_4_2 (AY496874), BDH02_7_02 (AY496877), DENV1 (M87512), DENV4 (AY618992).

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